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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: BvcZR3 and BvHs1 pro-1 Genes Pyramiding Enhanced Beet Cyst Nematode ( Heterodera schachtii Schm.) Resistance in Oilseed Rape ( Brassica napus L.)
doi: 10.3390/ijms20071740
Figure Lengend Snippet: Generation and identification of transgenic Hs1 pro-1 and cZR3 plants from hypocotyle explants of B. napus cv. Zheshuang 758 by A. tumefaciens mediated transformation. ( A , B ) Diagram of T-DNA region of the binary vector pAM194- Hs1 pro-1 ( A ) and pAM194- cZR3 ( B ). LB, T-DNA left border; RB, right border; P35S, Cauliflower mosaic virus (CaMV) 35S promoter; NOS, nopaline synthase terminator; MCS, multi clone site including Xhol restriction site; Hs1 pro-1 , Beta vulgaris Hs1 pro-1 gene open read fragment sequence; cZR3 , Beta vulgaris resistance gene sequence; GUS , β-glucuronidase report gene; NPTII , neomycin phosphotranferase gene for kanamycin resistance. ( C ) Callus induction from hypocotyl explants after co-cultivation with A. tumefaciens . ( D ) Shoots regenerated on SIM. ( E ) Shoots elongation on SEM. ( F ) Shoots rooted on RM. ( G , H ) PCR assay for transgenic Hs1 pro-1 plants ( G ) and cZR3 plants ( H ) M means 1 kb DNA ladder; P means Hs1 or cZR3 plasmid DNA, W means non-transgenic plant DNA; Hs1-1, Hs1-2 and Hs1-3 mean T0 independent transgenic Hs1 pro-1 plants; cZR3-1, cZR3-2 and cZR3-3 mean T0 independent transgenic cZR3 plants. ( I ) Southern blot assay for T0 independent transgenic cZR3 and Hs1 pro-1 lines, P1P2 means Hs1 and cZR3 plasmid DNA, respectively; 1, 3, and 5 mean T0 independent transgenic Hs1 plants; 2, 4, and 6 mean T0 independent transgenic cZR3 plants.
Article Snippet: The multiple induced shoots were isolated and transferred to
Techniques: Transgenic Assay, Transformation Assay, Plasmid Preparation, Virus, Sequencing, Southern Blot